Given the published roles of the H3K4 methyltransferases in transcriptional activation and/or maintenance, we were surprised to find very few genes down-regulated more than about twofold in absence of Mll2 and only one gene whose expression completely relied upon Mll2.
Pma1 protein level increases almost twofold in the absence of Sir2.
Moreover, SFRP3 mRNA was downregulated more than twofold in the absence of promoter hypermethylation in 71% of HCCs (17 of 24) (Table 4).
Autonomy is conceptualized twofold as (a) absence of disability in daily life and dependence on care, and (b) perceived self-determination.
(D ) Percentage of genes affected more than twofold in the absence of TAF9B as determined by RNA-seq analysis in Taf9b KO EB-MN differentiated for 6 days.
Comparison of gene-chip data revealed only few candidates, including Fbxw10, Fgd6, Hist3h2a, Hmha1, Igf1R, Lyrm7, RhoBTB1, Slc25a13 and Zfp39, that were deregulated more than twofold in the absence of Caspase-2 or RAIDD when compared with wt (Supplementary Figure 2).
Wintour's reasons for returning to the London shows after a few years' absence are twofold.
In addition, the mean baseline MUA in sensory zones was approximately twofold higher in the absence of isoflurane: 4.9±2.4 s−1 (with isoflurane, n=9 animals) and 11.9±4.6 s−1 (without isoflurane, n=10 animals) (mean±s.d., P≈6.5 × 10−4, Wilcoxon rank-sum test).
The expression level of these genes showed at least a twofold reduction in the absence of Relish when compared to control Drosophila S2 cells.
In total, we found that 297 gene transcripts were altered by at least twofold or greater in the absence of p53.
Several hypotheses have been presented in order to explain the absence of the twofold advantage [ 12, 13] many of them based on the discrimination ability and the efficiency of males during mate choice of sexual reproduction [ 14- 17].
