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Although there were no significant associations between COL27A1 and Achilles tendinopathy, a GCA haplotype (a set of genetic variants located on a single chromosome) constructed from one COL27A1 polymorphism (rs946053) and two TNC polymorphisms (rs13321, rs2104772) showed significant association with Achilles tendinopathy [30].
These two TNC types share a common embryonic origin, but they exert different functions [ 63].
Previously, we have identified two TNC isoforms that consistently and specifically are up-regulated in invasive breast carcinoma as well as in a subset of pre-invasive ductal carcinoma in-situ: one containing exon 16 (TNC-16) the other containing exon 14 plus 16 TNC-14/16 TNC-14/16
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Transient transfection was used to determine the direct effects of TNC isoforms (TNC-S, TNC-9/14/16, TNC-14/AD1/16) on invasion in the three TNC null cell lines (MCF-7, T-47 D, ZR-75-1) and in two cell lines with endogenous TNC expression (MDA-MB-231 and GI-101).
Mice were transfused with 3.5 8 million TNCs or 150,000 Lin- cells or 150,000 Lin+ cells.
In six TNC-AD1-positive breast carcinomas, the TNC-AD1 antisense probe localised to tumour cells and not to stromal cells; and in the associated myoepithelial cells around large histologically normal ducts, there was a lower level of expression detected.
The troponin (Tn) is a ternary complex consisting of three subunits TnC, TnI and TnT; molecular disruption of the Tn complex has been recognized as an attractive strategy against neuropathic pain.
Two contigs encoding TnC homologues were found.
In Lethocerus, two isoforms of TnC coexist in the same myofibril, F1 and F2, in a ratio of ∼10 1 (24, 24).
We previously identified induction of two intermediate-sized TNC isoforms in breast cancers [ 24] and showed that these isoforms can enhance tumour cell invasion [ 25]; a similar change was demonstrated in ovarian carcinomas [ 26].
In the flight muscles of Lethocerus, a giant water bug often used as a model system, there are two isoforms of TnC, F1 and F2, present in an approximately 10 1 ratio.
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