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To demonstrate causality, we have compared the in vivo behaviour of two TCC cell lines, one transfected with the TP gene (RT112-TP), the other an empty vector control (RT112-EV).
Thus, a continuous sequence such as (TCC 6TCT TCC 5 (as in fosmid 14K06, SSRs D and E) would be counted as two SSRs by SSRIT because the two TCC tracts are interrupted by a TCT.
Serine for example has two optimal codons, TCT and TCC, and if I would count one TCT, two TCC and five non-optimal codons in a gene, FOPTCT = 1/(5+1) and FOPTCC = 2/(5+2/.
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Interestingly, two TCCs (TCC4-1 and TCC7-5) displayed a Tc2 phenotype, releasing mainly Th2 cytokines.
Three TCCs (TCC8.1-2, TCC21-1, and TCC17-1) displayed a Th2 phenotype releasing mainly Th2 cytokines, two TCCs (TCC5.2-1 and TCC8.1-101) had a Th1/2 functional phenotype and only TCC8.1-35 TCC8.1-35mainly Th1 cytokines.
Th2-associated genes were upregulated in these two TCCs while Th1, Th17 and Treg-associated genes were downregulated (Fig. 3B).
Two TCCs generated from the CSF and present in L-III (TCC13.1-13 and TCC21-1) were also found in L-I although not among the 25 most frequent clones, but ranking lower regarding frequency (Table 4).
Either three TCC codons or two AGC/AGT (AGY) codons in the wild-type sequence of sinR were switched to remaining serine synonymous codons.
The three TCC codons and the two AGC/AGT codons (switched in Figure 1 figure supplement 2 ) are highlighted in green and blue respectively.
First, endogenous activity of the WNT/ β-catenin pathway was determined in proliferating NUEC and seven TCC lines under optimal growth conditions.
From these results, we concluded that five out of seven TCC cells were sensitive to metabolites of 5-FC after infection with OncovexGALV/CD (in the presence of 5-FC).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com