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In these two SFM modes the true topography is directly obtained by simple substraction of the topography and error signal data: z true = z fb - Δ I err (6).
The analyses of glucose, glutamine and amino acids (data not shown) have not revealed any shortage during the virus production in the two SFM and two viruses tested.
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Each test was repeated twice in two media (SFM and SCM).
This was tested via gene and protein expression after one or two passages in SFM and compared to hFK cells cultured in SCM.
Briefly, the cells were cultured for two days in SFM medium (serum free medium; DMEM/F12, 1% ITS, 1% BSA) supplemented with 4 nM activin A, 50 μM 2-mercaptoethanol, and 2 ng/mL bFGF.
For the SDS-gel analysis followed by Coomassie staining, α1-PDX (5 µg, 10 µM) was co-incubated for 15 min at ambient temperature with furin (0.5 µg, 1 µM) in either PBS or the Sf-900 II SFM medium.
For protein expression, 2.5 ml of viral stock was used to infect approximately 2×106 adherent sf9 cells in 400 ml of sf-900 II SFM serum free medium (Gibco) and cultured at 27°C for 48 h.
We have compared five commercially available SFM for the microcarrier based cultivation of Vero cells.
To inhibit IGF-1-stimulated signaling, cells were pretreated with a kinase inhibitor in SFM for two hours prior to IGF-1 exposure.
For each group, kids were fed one of three diets containing SBM, SFM, or SFM supplemented with fibrolytic cellulases enzyme (sunflower meal with enzyme, SFME).
As mentioned earlier, JRS maintains the four function blocks (RM, SFM, JRR, and RMH) for which various JRS control messages are exchanged among the devices.
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