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Two restriction sites were created for those mutations in each segment, Fsp I and Dra I, respectively.
Two restriction enzymes were used: BsrI, which cuts wsp amplicons in wVulC but not in wVulM, and MFeI, which cuts wsp amplicons in wVulM but not in wVulC.
Two restriction digestion enzymes were used (NlaIII, Sau3A).
Two restriction enzymes were used to generate sequencing tags.
Two restriction patterns, which were called M. albus 1 and M. albus 2, were revealed from the analysis of 36 colonies using two restriction enzymes.
Two restriction sites (Age I and Xho I) were shown in italic.
Two restriction enzymes were used to digest the genomic DNA: EcoRI and HindIII.
Two restriction sites of HindIII and EcoRI resided within the BaeI site) for the mutants' initial screening with colony PCR.
HindIII and NlaIII were chosen as two restriction enzymes to digest the genomic DNA and prepare the Illumina libraries.
And so they're going to scale with heterozygosity, and they scale with the combined length of the two restriction sites.
About 250 ng of genomic DNA is digested with one of two restriction enzymes and ligated to adaptors recognizing the cohesive four base overhangs.
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