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Two NSG mice receiving no cells were used as controls.
Two NSG mice receiving no cells were used as controls for in vivo imaging.
On the basis of those findings, we coinjected HS5 cells overexpressing CD146 with marrow cells from two patients with RAEB-2 and RAEB-1, respectively, into two NSG mice each.
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Unexpectedly, only ten A549 cells formed a tumor in one of the five NSG mice.
Tumors developed in two of the five NOD- scid mice (40%) and in all the five NSG mice (100%).
Tumors developed in two of the five NOD- scid mice (40%), while tumors formed in all the five NSG mice (100%).
When 10 EBcellslls were injected, no tumors developed in any strain other than NSG mice, while tumorigenesis was achieved in all the five NSG mice (100%, P=0.0079) within 9 weeks.
For this purpose, MCF-7 cells expressing either the conditional Mdmx KD or control lentiviral vector were orthotopically injected into contralateral mammary fat pads (numbers 4 and 9) of six NSG females and mice were treated with DOX through an IP injection followed by DOX-supplemented drinking water.
For transplantation into secondary recipients, bone marrow and spleen cells were collected from the three primary NSG recipients and sorted on the basis of expression of human CD45 (containing variable numbers of CD34+ cells).
Human PBMC (2 × 10) were injected into the peritoneal cavity of six to eight week old NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJmice, The Jackson Laboratory, Bar Harbor, ME, USA) in 200 μl of PBS.
For bone metastasis model two cohorts of NSG mice (castrated and intact) received orthotopic injection of VCaP-Luc cells into the bone marrow cavity of left tibia.
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