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Eumetopina flavipes were collected for genetic analyses from S. officinarum and S. 'hybrids' grown at nine TS and two NPA communities; these communities were termed locations (Table 1).
The deduced amino acid sequences of zebrafish aquaporins contain six predicted transmembrane helices and two NPA boxes that are the hallmark of the MIP superfamily [ 31].
All three contained the classical footprint of aquaporins: two NPA motifs, cysteine 181 was not conserved and only CL138 had the consensus site for N-linked glycosylation.
The two major constriction filters corresponding to the two NPA motifs and the ar/R filter were shown in Figure 1.
We hypothesize that isoforms lacking one of the two NPA or NPA-like motifs characteristic will have impaired or abolished water transport function.
Furthermore a positive or negative relative difference between two NPA scores denotes the same relative difference in the magnitude of the activity of the biological entities represented by the HYP.
Similar(47)
We therefore conducted analyses based on identification of pathogens (a) in one or other or both of the two NPAs collected before the age of 3 months and (b) only in NPAs collected at age 1- < 3 months.
Clinical specimens for examination included one NPA, three GA and three expectorated or three induced sputum specimens.
Eighty-seven NPA RT-PCR positive patients and 33 RT-PCR positive patients handserologic tests on their paired serum samples 10–14 days apart.
All four NPA methods demonstrated dose- and time-dependent decreases in the E2F1-direct HYP score at the 4 hour, 6 hour, and 24 hour time points.
For example, the four NPA methods supported the same time- and dose- dependent NF-κB activation in response to TNFα, whereas only Strength and GPI suggested NF-κB activation in response to CDK inhibition.
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