Similar to stress conditions, these data did not allow plastid transcriptomes and genes to be classified into two groups and two NEP and PEP determined clusters, respectively (Supplementary Fig. S2 and Table S6).
The two NEP clusters appeared reciprocally monophyletic in the cyt-b data and in the two-gene concatenated dataset, which was not evident from the CR data alone (Additional file 4: Figure S2).
There was no significant differentiation between the two NEP lineage samples in terms of AMOVA apportionment of nucleotide diversity (ΦST = −0.002 for the concatenated dataset), neither among the four samples of the Asian coast and the Bering Sea that made up the NWP lineage (ΦST = 0.005; Table 3).
Because of data limitations regarding equivalent land use data across space and time, the analyses were isolated to the 12 NEPs located in the Northeast Region (NE) and three NEPs located in the Gulf Coast Region (GC) for a total of 15 NEPs (Fig. 2).
Seven NEP-deficient mice and 7 age-matched wild-type controls received a telemetry implant (TA11-PA20; Data Stiences International, St Paul, Minnesota, USA) in the carotid artery as described earlier by Gross et al. [38].
Subjects were equally distributed across the six NeP conditions, with the majority of subjects in each condition characterized by moderate or severe pain (Table 2).
Of the six NeP conditions studied, post-trauma/post-surgical NeP and chronic low back pain with NeP accounted for the highest proportions of patients with moderate or severe pain, which may drive the HRU and total economic burden.
Data for this analysis were obtained during a cross-sectional, observational study on the burden of NeP across six NeP conditions: painful diabetic peripheral neuropathy, 4 human immunodeficiency virus-related peripheral NeP, 18 posttrauma/postsurgical NeP, 5 spinal cord injury-related NeP, 6 chronic low-back pain-associated NeP, 19 and small-fiber neuropathy.
Potential subjects with one of the six NeP conditions of interest, related to human immunodeficiency virus, post-trauma/post-surgical NeP, spinal cord injury, chronic low back pain, painful diabetic peripheral neuropathy, and small fiber neuropathy, were identified by site staff when they presented for routine office visits.
6, 9 One of the only studies that investigated HRU among subjects with a broad range of nine NeP conditions was conducted over a decade ago; this medical claims analysis reported total annual direct health care charges to payers (US$17,355), which are generally considered to be substantially higher than health care costs.
