The two NCP-binding regions of HMGN2 are separated by a rigid proline-rich linker.
Ten thousand simulations were run for all subjects and for each comparison (Left CP versus Right CP, Left CP versus NCP, and Right CP versus NCP).
However, the six NCP molecules that form the hexameric capsomers around the icosahedral 2-fold axes made extensive contacts.
Contrary to the organization of the E1-E2 glycoproteins, where there are extensive inter-heterodimer contacts within the trimeric spikes, there were few contacts among the three NCP molecules around the icosahedral 3-fold axes and no contacts among the three NCP molecules around the quasi-3-fold axes.
Intragenic recombination among some samples is likely: A discordance in the alignment can be resolved by postulating a recombination in three of the four ncp GS haplotypes of A. nobilis-2x around the 89th polymorphic site (Additional file 1: S-Figure 1).
In order to compare the relative stability of the three NCPs, the ionic strength of the solution was increased and the resulting species analyzed by EMSA (Additional file 1: Figure S8B,C).
These temporal parameters were subjected to ANOVA analysis using condition (Left CP, Right CP and NCP) as the independent variable for within-participant and within-item comparisons.
In order to observe the general gesture behavior across participants, we computed the average matrix and the corresponding average tolerance ellipse for each of the 3 conditions (Left CP, Right CP and NCP).
One might ask why there was a difference between Left CP and NCP, but not between Right CP and NCP.
The difference in the orientation between Left CP and Right CP (39.9°, p = 0.022) and between Left CP and NCP (27.6°, p = 0.025) were statistically significant according to Monte Carlo simulations.
Ten subjects from each of the three groups (NCP, CRP and CWP) were randomly selected and included in this study.
