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Samples from sheep belonged to two MST types: ST28 was detected in a sample with AG MLVA genotype, and the proposed ST37 was described in samples with AF MLVA type.
A first map of DIP was based on 73 plants of a segregating population, 34 amplified fragment length polymorphisms (AFLPs) resolved by a bulked segregant analysis and linked alleles at two Mst loci [ 38].
A significant linkage of the rDNA locus to two Mst loci, Mst53 and Mst78, that were linked to diplospory, suggest a physical location of DIP on one of the nucleolar organizer region (NOR) chromosomes [ 44].
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Figure 3 shows the two signals x t) and y(t) and their related MSTs. Figure 4 shows the cross-MST of the signals x t) and y(t) corresponding to the two MSTs in Figure 3c,d.
Key parts of the commented Matlab® code written to implement this method are given in Appendix 2. Figure 7a represents the real part of the cross-MST of the two MSTs in Figure 6.
Comparison of MST data with previously determined IS6110-RFLP data indicated that 48 M. tuberculosis isolates (51.61%) clustered in the same way using MST and IS6110-RFLP typincludinguding 28 isolates clustered in nine MST and 13 IS6110-RFLP profiles; and 20 unique isolates.
Sixteen MST genotypes were found, 5 previously identified in cats and 11 new.
Contig assembly and analysis revealed a minimum of 18 expressed loci across the seven MST subfamilies.
Six MST genotypes belonged to human and cat strains, including 2 predominant MST genotypes (14 and 35).
Homologs of all seven MST subfamilies were present in land plants at least 400 million years ago.
Alignments of each translated EST sequence with its best match Arabidopsis gene were reviewed to confirm identity of each EST as a member of one of the seven MST subfamilies.
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