Sentence examples for Two lcm from inspiring English sources

In two LCM samples, unconverted genomic DNA comprised more than 10% of the total DNA, indicating a poor conversion.

Three commonly used LCMs that vary in size were used to formulate and evaluate the effectiveness of nine LCM blends.

We tested this hypothesis by comparing the seven LCM cell populations.

Four LCM caps were required for MA (mean cell count per cap = 6,202.2 ± 223.2) and eight caps for MG sections (mean cell count per cap = 994.2 ± 42.5) to provide sufficient amounts of RNA for downstream microarray analysis (Table 1).

The majority of those absent from the three LCM samples had low expression in whole anthers; only 235 (3.5%) are above the first quartile and 14 (0.2%) are above the median, including two TFs.

The three LCM tissues were screened by qualitative end-point RT-PCR to investigate tissue-specific expression of each gene within the seed at 5 dap, which confirmed that all three genes are indeed expressed in Arabidopsis thaliana seeds (Additional file 7 Figure S2).

First, the average probeset expression value across all six bulk normal samples was compared to the average probeset expression value across all six LCM normal samples, and likewise for the cancer samples, identifying 387 probesets in normal tissue and 500 probesets in cancer tissue with significantly varied expression levels.

Adding the 14 transcripts above the median to the 811 downregulated in all three LCM samples, we can assign 825 transcripts to non-lobe tissues (vasculature and connective cells); these are the first presumptive markers assigned to these non-lobe cell tissues.

The four LCM samples also showed clear 28 rRNA and 18 rRNA bands, but the 28S/18S ratios were different: 1.75 for the unprocessed cell pellet (A), 1.84 for MGP, 4.76 for H&E, 0.71 for NISSL, 2.05 for IF, indicating that a small amount of degradation appeared in the Nissl RNAs the only sample with a ratio less than 1.7.

Open image in new window Fig. 8 Optical microscopy images of the three LCMs.

This time, six LCMs and six LCVPs were used instead of the LVTs.