Sentence examples for Two gsc from inspiring English sources
Self-renewal was assessed using a limited dilution assay, which demonstrated that treatment with CBD+ERA significantly downregulated GBM stem cell frequency in two GSC cell lines.
The two GSC lines (#83 and #61) in which TMZ and PARPi association did not result in synergistic effects were the most resistant to PARPi monotherapy and were poorly responsive to TMZ as well.
After inducing GSC clones at a low frequency (i.e., dominantly single GSC clone/testis), symmetric outcome can be assessed by scoring the frequency of 'doublet' clones (i.e. two GSC clones are juxtaposed each other) (Salzmann et al., 2013).
Treatment with the PARPi enhanced TMZ efficacy in all but two GSC lines and the potentiating effect directly correlated with sensitivity of each cell line to the PARPi used as single agent.
The two GSC lines assayed displayed marked resistance to most of the compounds tested, whereas inhibition of PKC (rottlerin and Ro 31 8220) or ERK2/adenosine kinase (5-iodotubercidin), PDGF-R (tyrphostin 9), and EGF-R (erbstatin analog) inhibitors yielded significant antiproliferative results.
The first method is to generate two GSCs through a symmetric cell division, and the other is to generate one GSC and one non-GSC through an asymmetric cell division.
(B ) In the c587>>UAS-GFP germarium containing two GSCs (spectrosomes indicated by arrowheads) close to cap cells, one CB and a few differentiated cysts are surrounded by GFP-positive ISCs.
For D. simulans, we used the theta range we observed across the eight GSC loci in this study, which ranged between 0.003 and 0.04 per site.
In all the three GSC lines, CHX was protective against celastrol-mediated toxicity, confirming our previous findings from U251N and U343.
Eight GSC lines were successfully established by serum-free clone formation and began floating and propagating when cultured in serum-free medium containing EGF/bFGF/B27 (NSC medium).
Seven GSC lines showed TMZ IC50s between 200 and 600 μM that are markedly above the peak plasma concentration reached in cancer patients.
