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Two FSC curves were obtained, one from maps calculated from two halves of the data set, another using the modeled atomic coordinates.
The two FSC curves nearly overlap, and we observe significant correlation beyond the resolution used for refinement (indicated by a vertical dashed line in Figures S3 B and S3D), demonstrating that the model has predictive power and has not been overfitted.
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Two FSCs are maintained within a structure at the tip of each ovariole, called the germarium (Margolis and Spradling, 1995).
The results indicated that genetic differentiation among groups was maximized at six groups (FCT = 67.06) while differentiation between populations within groups dropped below zero (FSC = −0.15).
Last year, Tetra Pak became one of FSC's largest multi-site certified suppliers by obtaining certification for 92 facilities worldwide, and sold 43.7 billion FSC-labeled cartons.
Hierarchical analysis of molecular variance (amova) was conducted to test whether a significant proportion of genetic variance was partitioned into components attributable to differences among wild, captive-spawned, and captive-reared stocks (i.e., wild-caught eggs were the source) (FCT, ΦCT), among samples within these three groups (FSC, ΦSC) and among all samples (FST, ΦST).
We compiled written information from three larger FSC-certified forestry companies combined with semi-structured interviews with forest management personnel at regional offices to map what data and information the companies collect and use.
To determine the possible presence of cell doublets or clusters in GFP-high population, we estimated cell size and granule-density between these two populations by FSC and side scatter, respectively and found no difference (ESM Fig. 1).
What we emphasize here is, first, that none of the four optical parameters (FSC-A, FSC-W, SSC-A, and 450/50-A) we investigated in four cell types provided the best indication of cell volume for all cell types and, second, that a combination of light scattering and fluorescence parameters was likely to approximate cell size of any given cell type better than any individual optical parameter.
Twenty thousand light-scatter (FSC versus SSC) gated events were analysed, and results were expressed as percentages of total lymphocytes.
(C ) Fourier Shell Correlation (FSC) between two independently calculated maps from two halves of the data.
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