Sentence examples for Two dgc from inspiring English sources

The two dGC values obtained for each gene pair were plotted along chromosome pairs using a sliding window of 11 genes with a step of 1 for representing a moving average of 11 adjacent genes along each chromosome.

Knockout mutations in the two DGC genes yegE and ydaM strongly reduce CsgD and therefore curli expression, whereas mutations in the PDE genes yhjH or yciR generate the opposite phenotype (Weber et al, 2006; Pesavento et al, 2008).

Differential stability, localization, or protein-protein interactions of Y3730 and HmsT in the two environments could also contribute to differential activity of the two DGCs in vitro and in the flea.

In this study, we evaluated the role of the ten known or putative DGC- and PDE-encoding genes of Y. pestis in regulation of the biofilm phenotype, and show that the two DGCs of Y. pestis make different, environment-dependent contributions to biofilm formation.

In summary, our results confirm that only three c-di-GMP metabolizing proteins– two DGCs (HmsT and Y3730) and one PDE (HmsP) are involved in regulation of biofilm formation in Y. pestis, and demonstrate that the role of Y3730 in biofilm production is much greater in the flea than in culture media.

Our results confirm that only one PDE (HmsP) and two DGCs (HmsT and Y3730) control c-di-GMP levels in Y. pestis, indicate that hmsT and y3730 are regulated post-transcriptionally to differentially control biofilm formation in vitro and in the flea vector, and identify a second c-di-GMP-regulated phenotype in Y. pestis.

In this study, we provide further evidence that hmsT, hmsP, and y3730 are the only three genes involved in c-di-GMP metabolism in Y. pestis and evaluated the two DGCs for their comparative roles in biofilm formation in vitro and in the flea vector.

Although c-di-GMP levels are known to regulate the expression of virulence factors and pathogenesis in other bacteria, loss of the two DGCs did not significantly affect pathogenesis in a mouse model of bubonic plague (Fig. S2), suggesting that c-di-GMP does not control the expression of essential virulence factors in Y. pestis.

The cells expressing these latter two DGC-proteins had the morphological characteristics of neurons.

There was partial overlap (yellow) between all three DGC-proteins (red) and MLC1 (green).

Mutations were found in the upstream region of three DGC-encoding genes.