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Two coa of strains isolated from patients associated with pigs were sequenced and typed SC type VIIb.
Two CoA biosynthesis related genes (Cbei_2609-2610) also demonstrated higher expression in R13.
Formation of ketones also results in the liberation of two CoA molecules.
Introduction of coa allele in the cell presumably by phage transduction or transformation and recombination between the two coa might have occurred in the past.
We also used the nucleotide sequences of two coa partially-sequenced strains isolated in Hokkaido in Japan [20] and of fourteen whole genome-sequenced strains [21] [31] obtained from the DDBJ/EMBL/GenBank databases.
This unveiled the identification of the bvaA, bvaB1, and bvaB2, genes that encode a CoA ligase (BvaA) and two CoA ammonia lyases (BvaB1 and BvaB2).
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Two CoAs associated with the MNs in the HMIPv6 protocol: RCoA and local care of address (LCoA).
In this study, 29 strains isolated from cats were analyzed by M-PCRs and eighteen coa of them were sequenced.
Throughout the whole genome, there are 11 genes that potentially encode racemases, including six amino-acid racemases, three CoA racemases, and two mandelate racemases (see Additional file 1: Table S6).
The nucleotide sequences of three coa genes were deposited in the DDBJ/EMBL/GenBank database under accession numbers AB742446 (strain O-2), AB742447 (strain N-1) and AB787218 (strain P-1).
About 19 biosynthetic enzymes are needed to form taxol from GGPP including taxadiene synthase (TS), eight cytochrome P450 oxygenases, three CoA-dependent acylations and several other enzymes [ 33- 35], as well as several undefined enzymes in the taxol biosynthetic pathway [ 34- 36].
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