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We propose that the re-replication fork may spontaneously abort, yielding two chromatid fragments terminated by double-strand breaks (DSBs); fusion of the DSBs restores the chromosome linearity and generates CIs containing Ac/ fAc and their flanking sequences at the duplication breakpoints.
A single telomere signal at the end of a chromosome may represent the juxtaposition of two chromatid ends or the fusion of two sister chromatid telomeres; it is not possible to distinguish between these possibilities when the single signal is seen on the short arm of the chromosome.
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Sutton had observed that, during meiosis, each chromosome (consisting of two chromatids) becomes paired with a physically similar chromosome.
In meiosis the two chromatids making up each chromosome remain together, so that whole chromosomes are separated from their homologous partners.
Cell division then occurs, followed by a second division that resembles mitosis more closely in that it separates the two chromatids of each remaining chromosome.
If the inverted segment contains the centromere (i.e., the point where the two chromatids are joined), the inversion is said to be pericentric; if not, it is called paracentric.
Cells were treated with DMSO or bDMC for 24 h, and Giemsa-stained metaphases were analyzed: while control cells did not show chromosome abnormalities, we detected an average of two chromatid-type aberrations per cell, in particular gaps and breaks, following bDMC administration.
Anaphase/telophase (D-F) chromosomes split into two chromatids.
This results in four gametes, each carrying two chromatids that are not sister chromatids.
(E ) The two chromatids fuse to form a new chromatid containing a Composite Insertion.
Further analysis of these three events using PCR and sequencing showed that they indeed involved three chromatids (Additional file 1 - Figure S5), suggesting that DSBs were generated in two chromatids, and that the recombinogenic broken ends from the DSBs interacted with the other two chromatids during the recombination process, in four-chromatid events.
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