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A GGGGSGGGGSGGGGS linker was used to separate the two CFP sequences.
If the proteins of interest interact, and the two CFP fragments are brought into the proper orientation, then they will reassemble and restore fluorescence.
Then, we appended two CFP proteins to increase its molecular weight beyond the passive diffusion limit and thus reduced potential leakage of TEV into the nucleus prior to decaging.
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They assured one CFP audience after another that the technologies of information collection were benign.
Since the ANN model can be trained from iterations, it predicted CFP with high accuracy inspite of the presence of nonlinearities, i.e. the average of the mean R2 value for the three CFP was found as 0.96.
Two persons of sixteen (CFP 12%) died in an outbreak associated with homemade cheese in New Mexico in 1983 [ 17].
These results demonstrate that all recombinant clones tested expressed only one CFP-tagged gene transcript.
RD1 selected peptides were used as follows: a pool of the two ESAT-6 peptides (at 10 µg/mL each), a pool of the three CFP-10 peptides (at 2 µg/mL each).
RD1 selected peptides were used as follows: a pool of the two ESAT-6 peptides (at 10 μg/mL each) and a pool of the three CFP-10 peptides (at 2 μg/mL each).
When RalA is activated, the GTPase binds to the RalBP1 fragment and triggers a conformational change in the molecule that enables a FRET [21] between the two fluorophores, CFP and YFP.
Among 11 patients hospitalized in Hong Kong because of septicemia from 1982 to 1986, two died (CFP 22%) [ 10].
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