In both sequences, two CUB domains, an EGF-like domain, two CCP domains, and a trypsin-like serine protease domain were identified.
In contrast, the genes from hymenopteran insects, e.g., bees and ants, dipteran insects, e.g., mosquitos, and coleopteran insects, e.g., red flour beetles, include two CCP domains.
The N. lugens clotting factor C like gene consists of a signal peptide, three consecutive low-density lipoprotein receptor class A domains (LDLa), two CCP domains and a serine protease domain with the catalytic triad.
The electrostatic bindings of the three CCPs with dsDNA/EB complex resulted in displacement of EB from dsDNA to the solution accompanied by the quenching of EB fluorescence.
The interactions among the three CCPs and double-stranded DNA (dsDNA) were studied using ethidium bromide (EB) as the fluorescent probe.
Compared with LP2′, HBP1′ exhibited increased water solubility and fluorescence quantum yield despite its lower charge density, and HBP2′, with the similar charge density as LP2′, showed the best water solubility and the highest fluorescence quantum yield among the three CCPs.
However a check of the 47 outgroup potyvirus cCPs using the RDP3 package of programs found only five cCPs with tentative evidence of recombination, and that was given by only with a single method, Siscan, out of the seven in RDP3 and by PHYLPRO, and only one cCP, that of onion yellow dwarf virus, gave tentative evidence of recombination by two methods.
Although the three CCPs shown in Figure 4 originated from the same seed, their connectivity profiles diverged at cliques #17 {ESR10, ESMAD4SMAD3, SMAD3, CBP, AR, CTNNB1}, #39 {BRCA1, EP300, ESR1, SMAD3, SMAD, TP53, SP1}, and #GC1 {EGFR, GRB2, PIK3R1, PTPN6, SHC, SRC, ESR1}.
The D strain cCP sequences came from isolates collected throughout Europe, Eurasia and the Americas, whereas the M strain cCPs came only from Europe, and there are also four cCP sequences from three other distinct strains.
SUSD4b is a smaller isoform (27 kDa) consisting of three CCP domains and a region of unknown homology.
