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Metabolites were identified through retention time and UV/visible light spectrum compared to authentic standards, followed by a total quantification with dose-response curves72.
Fungal production of small concentrations of alkanes, which are undetectable by the PTR-MS, and small errors in calibration of the CO2 detector and PTR-MS may also contribute to errors in total quantification.
The eXpress was executed in the streaming mode, to save the total quantification running time.
The total quantification assay was composed of two methylation-unspecific oligonucleotides and a scorpion primer unspecific for DNA methylation.
Real-time PCR assays were comprised of two independent reactions: a total quantification assay for quantification of total input DNA and an HM assay [ 21] for quantification of methylated target template.
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Additionally, the total VOC measurements can be compared to less certain total quantifications performed with other instruments, such as PTR-MS, to identify discrepancies in the system.
Commonly, elemental studies in Romania have suffered from limited sampling numbers (n = 5 10) and/or incomplete digestion offering only semi-total quantification of elements using traditional, laboratory-based techniques.
The isotopes used in this study were as follows: Zn and Zn for total zinc quantification, Cu and Cu for total copper quantification, Ni and Ni for total nickel quantification, and Cd and Cd for total cadmium quantification.
The following combination was used for total TrkB quantification: TrkB capture with monoclonal anti-TrkB (1∶1000); total TrkB quantification with polyclonal anti-TrkB (1 µg/ml).
The reproducibility was determined on the total linear quantification range of both curves confirming the accuracy of the technical set-up in the complete ranges of quantification over time.
The intensity of all bands per lane was quantified and the total band quantification was set to 100%.
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