Out of a search space of 2 × 1012 sequences, the calculation selects a point mutant of the native protein as the top solution (experimental Kd = 17 μM) and the native protein as the second best solution (experimental Kd = 210 nM).
Among the many reasons for the currently limited research output in melt electrospinning (MES) is that the fabrication of a well-designed melt-based electrospinning devices is technologically and scientifically more challenging than assembling a laboratory-scaled bench top solution electrospinning (SES) machine.
Fig. 13 Geometry (top), solution of Poisson's equation with iso-contours (bottom).
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The two plates, once loaded, can be combined by slipping them together so that specified volumes of the top solution diffuse into the isolated microwells (Fig. 21) [36].
The first 50 mL was the bottom solution, the intermediate 100 mL was the middle solution, and the last 50 mL was the top solution.
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Figure 4 XPS spectra of CdS nanorods prepared (40 mg cadmium acetate in 3 mL TOP and 24 mg S powder in 1 mL TOP at 260 °C for reaction 4 h) in TOP solution:a Cd b S c C d P Figure 5 FT-IR spectra of (a) pure TOP and (b) CdS/TOP core/shell nanorods.
Figure 1 CdS nanorods synthesized in TOP solution (40 mg cadmium acetate in 3 mL TOP and 24 mg S powder in 1 mL TOP at 260 °C for reaction 4 h): TEM image (a), HRTEM image (b), length (c) and width (d) distribution histograms of the nanorods.
To test the first scenario, we added freshly prepared hematin-saturated wet octanol to the top solution layer.
For controls, 10 µl of Top Solution and antibiotics (Kanamycin 50 µg/ml) were added to the disc.
