Sentence examples for To validate the changes from inspiring English sources

To validate the changes in metabolites in our dataset, we conducted a metabolomic screen using the metabolomic services at Metabolon, which utilizes internal standards to validate metabolites.

To validate the changes in mRNA expression, culture supernatant were collected after treated with PA for 16 h and ELISA analysis were performed.

To validate the changes in primary miRNA expression, we performed pri-miRNA TaqMan® qPCR on the four miRNAs that met all our criteria.

To validate the changes in EMT of MCs, the expression of the epithelial marker, E-cadherin (anti-E-cadherin; BD Bioscience, Bedford, MA), were identified [ 21].

To validate the changes in expression observed from the microarrays, we performed qRT-PCR for 12 Paupar-regulated genes with two additional Paupar-targeting shRNA expression constructs (Supplementary Fig S2B).

To validate the changes in gene expression in response to TNFα-induced MEK/ERK signalling determined by the microarray analysis, we identified the relative changes in transcript levels of the extracellular matrix components Agc1, Hapln1, and Col2a1, proteases Mmp-9 and Mmp-12, as well as the inflammatory cytokine macrophage Csf-1.

The purpose of the current study was to validate the change in thoracic impedance (dZ) derived respiratory signal obtained from four spot electrodes against incidental spirometry.

The data used to validate the change version of the questionnaire (STCD2-c) were obtained in telephone interviews carried out 8 weeks after discharge from hospital (in those cases where the blood glucose-lowering medication had been changed during hospital admission) and further telephone interviews 4 weeks later in which the questionnaire was repeated.

Immunoblotting was used to orthogonally validate the changes in expression of a subset of the ETC complex subunits (ATP5A1, UQCRC2, MTCO1, NDUFB8, and ATP5H) in the synaptic mitochondria during aging.

Further studies are warranted in order to examine in detail the cellular origin of the alterations observed in our analyses, to validate the observed changes using complementary approaches and to investigate possible genetic factors related to the observed alterations.

We tested the expression of seven selected genes by real-time RT-PCR, and were able to validate the developmental changes between P5 and P13 in six of them (see examples in Figure 3A).