Sentence examples for To pnp from inspiring English sources
When activity with other donors and acceptors was tested, the initial enzyme preparation cleaved pNP-β-d-glucopyranoside (Glc-pNP) and pNP-β-d-fucopyranoside (Fuc-pNP) to pNP and glucose and fucose, suggesting contamination with wild type BGlu1 β-glucosidase.
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Conversion to pNp at room temperature was measured during 10 min using an ELISA plate reader (BioRad) at a wavelength of 415 nm.
The assay was performed by adding appropriate dilutions of 20 μl of the secretome to pNP plates that had been previously preincubated at 37°C.
Black line represents response of MnO NPs/BCA/gold electrode to 0.1 M phosphate buffer (pH 7.0) and red line represents the electrochemical response of MnO NPs/BCA/gold electrode to PNP, and b cyclic voltammograms showing response of 1 mM PNP in PBS using MnO NPs/BCA/gold electrode at 50 800 mVs-1 scan rates.
Interestingly, although ERD in AB group seem to be of a comparable intensity to PNP and was weaker than of PWP group, all CSP classifiers showed higher classification accuracy for AB than for PNP group.
For example, a cutinase of Fusarium solani prefers pNP-butyrate to pNP-acetate as substrate, and cutinase-like enzyme of Cryptococcus sp. S-2 prefers pNP-caproate to pNP-butyrate and pNP-acetate (Kodama et al. 2009).
As compared PLA-MAC to PNP-MAC, the PLA-MAC outperforms by allocating separate and dedicated slots for non-emergency and emergency data in the CFP period.
Moreover, the E386G glycosynthase transferred glucose from GlcF donor to glucose, cellobiose, Glc-pNP, Fuc-pNP, pNP-β-d-galactopyranoside, and pNP-β-d-xylopyranoside acceptors, but little to pNP-β-d-mannopyranoside. Production of longer oligosaccharides occurred most readily on acceptors with an equatorial 4-OH.
We observed no peaks that were unambiguously attributable to PNPs, which were expected given the low atomic numbers of the constituent elements.
This is the first study to identify protein changes in response to PNP-GDEPT as obtained in OVCAR-3 cells using Shotgun Proteomics [ 47, 48].
