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While not unexpected, this result highlights the importance of removing or ameliorating impediments to passage up and down the rivers and through the Delta, and improving our understanding of environmental effects, including climate change, on ocean survival.
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This interval of time (capture time) ranges from less than one day (a single passage), up to 500 days, or even more.
Further, the invasive phenotype of selected cells has remained constant through multiple passages (up to 20) and through freeze-thaw cycles (up to 3 tested).
bPGCs were cultured (hereafter known as cPGCs) up to passage 30, as previously described [13].
hESCs were successfully cultured up to passage 25.
Under these conditions, cell phenotype is guaranteed up to passage 14 (Promocell).
Expression of IL-6 mRNA remained stable after this point up to passage eight.
In a long-term culture experiment, kidney cells were maintained for a high number of passages ‐ PKC2109 cells at least up to passage 71 and PKCm up to passage 50 ‐ showing only slight signs of senescence, whereas PFF and PEF could hardly be passaged more than 20 times.
The expression levels of these markers were slightly increased up to passage 5, but their relative amounts were still low.
In general, AD-MSC from younger sand rats up to age 12 months were passaged six to eight times; AD-MSC from sand rats older than 12 months were usually only passaged up to three times.
The cells were clonally expanded and serially passaged up to seven generations over 3 months, achieving an increase in cell number calculated to be up to 3,200-fold 3,200-fold
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