To further ascertain whether TFEB is a substrate of SIRT1, we carried out in vitro deacetylation assay with purified SIRT1 and acetylated Flag-tagged TFEB (Fig. S3E).
To further ascertain whether K116 site is acetylated, we generated single acetylation-deficient TFEB mutant (K-to-R mutation) by site-directed mutagenesis.
To further ascertain the formation of FLT3 drug-GNP conjugates and get supplementary information regarding the conjugation, DLS and zeta potential measurements were performed.
To further ascertain whether benefits of continuous infusion in critically ill patients do exist, a large-scale, prospective, multinational trial with a robust design is required.
To further ascertain these results, pleated structures of MFEC containing Pd/γ-alumina were tested for application in catalytic aircraft cabin air purification (ozone decomposition).
To further ascertain whether the model without the method effect due to the negatively corrected items was poorly specified, composite reliabilities with the correlated uniquenesses (see Additional file 4: Table S4) and without the correlated uniquenesses (not reported) were compared.
To further ascertain whether or not FC induces H2O2 removal, we also compared the effects of FC with CAT on the closed stomata induced by ABA and the level of H2O2 generated by ABA.
To further ascertain the proteasomal-dependent degradation of p62 by parkin, the Agt5 −/− MEF cells (Fig. S3), in which autophagic activity is abrogated, were employed to detect the p62 protein.
To further ascertain the chemical compositions of the nanowires, the as-prepared products were examined by TG-SDTA and Raman scattering spectroscopy in Additional file 3: Figure A3 and Additional file 4: Figure A4, respectively.
To further ascertain the formation of eccDNA in vitro we performed the reaction using artificial substrate as a template DNA.
To further ascertain the similarities between NLP and NB, a detailed morphological analysis was performed on the precipitates generated.
