Your English writing platform
Discover LudwigSimilar(60)
Thereafter, cells were ready to be passaged after 6/7 days culture, at 80%% confluence.
The feeder-free cultured BCs are able to be passaged for at least 30 doublings with no obvious morphology change.
The high number of chondrocytes needed for tissue-engineered cartilaginous implants requires the cells to be passaged repeatedly.
Once cells had grown to 90-100% confluency they were passaged into a 25-cm2 tissue culture flask and continued to be passaged into larger flasks until sufficient numbers were generated for storage.
Once the primary cell lines were established, cells from all tissue types (Table 1) grew well and were able to be passaged a number of times.
Cells were returned to the incubator until they were ready to be passaged.
During cultural maintenance, the cells do not need to be passaged.
Notably, MSCs derived from mouse and rat had to be passaged several times to achieve a high level of purity.
Somewhat surprisingly, these cells were able to be passaged, with no apparent morphological change and had normal population doublings.
Briefly, self-renewal was assessed by the ability of cells to be passaged in neurosphere culture at clonal density (5-10 cells/μl) for at least two passages.
Within 3-4 days, the large clumps of spheres became dark in the center, indicating that several cells were dying and that the neurospheres were ready to be passaged).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com