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To begin with, the dataset is restricted to assays containing between 8 and 25 molecules (inclusive).
The dataset was restricted to assays containing from 8to2525 (inclusive) unique molecules.
The electrochemical format and a custom, low-cost smartphone-based potentiostat ($20 USD) yielded comparable results to assays performed on a state-of-the-art electrochemical workstation.
Considering that the complete spectrum of linear and/or conformational immunodominant epitopes is yet to be identified, the use of the full-length antigen may be diagnostically advantageous compared to assays based on peptides.
Substrate cleavage was readily monitored and was linear for up to 4 h but enzyme concentrations of about ten-fold higher were required compared to assays using protein substrate LC3 or analogs thereof (such as FRET-LC3).
The anti-sticking operation of the valves simplify the working principle, reduce the reagents needed, and enhance the fluorescence intensity of the assays performed on chip by reducing non-specific binding compared to assays performed on plate.
Among the variety of biomimetic recognition schemes utilizing supramolecular approaches molecularly imprinted polymers (MIPs) have proven their potential as synthetic receptors in numerous applications ranging from liquid chromatography to assays and sensor technology.
Recent advances include the improvement of cryopreservation procedures to allow cell attachment, thereby extending the use of the cells to assays that requires prolong culturing such as enzyme induction studies.
When submitted to assays with different substrates and inhibitors, it showed selectivity for succinyl-l-ala-ala-pro-l-phenylalanine-p-nitroanilide and was almost completely inhibited by phenylmethylsulfonyl fluoride, behaving as a chymotrypsin-like protease.
These 8 films had been conditioned in deionized water for 14 days prior to assays.
Cells were harvested 48 h after transfection, and extracts were subjected to assays to determine the activity of reporter enzymes.
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