Assuming that five reads is a minimal depth to reliably determine the sequence of a haploid genome (K eightley et al. 2009), we see in Figure 3B that >97.8% of ycnbwsp_3-HE and 96.7% of ycnbwsp_2 are covered with five or more reads.
Depth profiles from acoustic tomographies were significantly different among substrates (ANOVA, F3,480 = 335.8, p < 0.001, Tukey HSD p < 0.001 except limestone and leaf) from a minimal depth profile on slate to a significant profile on bark, thus, we consider the effect of substrate in all further analyses.
Sequencing on each the SOLiD and Genome Analyzer platforms was completed independently, with assembly algorithms each requiring a minimal depth of 3 reads in order to confirm an allele call.
To trigger only a minimal unspecific physiological stimulus, sham acupuncture is applied with a minimal depth of needling (not exceeding 5 mm), avoiding real acupoints.
This indicates that the deployment of vertical arrays of trays with a minimal depth of 1.0 m may efficiently utilize the water column to grow out juvenile C. virginica.
> -wrap-foot> Here, we should note that these classifications should be considered as a minimal depth of conservation, as orthologs might be found by scanning additional species, different evolutionary groups, or finished genomes as they become available.
The samples were studied with a Leica multiphoton system using a titanium sapphire laser (excitation 800 nm with pulses in the 200 fs range) to obtain a stack of two-dimensional (2D) images to a minimal focus depth of 120 μm.
While the level of concordance for mutational analysis using this panel compared to classical Sanger sequencing is excellent, we could 'only' multiplex 5 samples on a 316 chip to reach a minimal read depth of 500× per amplicon.
We next ran an optimization identical to optimization 5 except that it constrained MZ dimensions to 30 × 30 rows, closer to experimental MZ dimensions, which led to a minimal increase of optimal pedigree depth to 9.65 (Table 1, optimization 6).
