Sentence examples for Through dideoxy sequencing from inspiring English sources

Through dideoxy sequencing, we identified four cases with at least one insertion or deletion (35delG/35delG, 35delG/167delT, and M1V/167delT in GJB2 and homozygous 1180_1187del8ins β-sat) in TMPRSS3), of which 2 had been previously reported and therefore had probes tiled on the array for their detection (GJB2 35delG and 1180_1187del8ins β-satine 1).

The presence of A457P was also determined through dideoxy sequencing.

Additionally, mice in the initial litters were also sequenced for the presence of A457P through PCR and dideoxy sequencing of a region spanning the A457P mutation (5′-CTATAGATGGGAGGCATGGAG-3′ and 5′-CAGCCCCAGAAGTAGTAGAAGG-3′).

The authenticity of all constructs was verified by dideoxy sequencing using ALF sequencer (Amersham Pharmacia Biotech, Piscataway, NJ).

Dideoxy sequencing was performed on an ABI 3730 Sequencer.

We could PCR-amplify 304 sequence variants; dideoxy sequencing revealed that all carried the predicted mutation (Supplementary Table 2).

The results obtained for tumor samples were 95% comparable to those obtained by dideoxy sequencing.

Dideoxy sequencing of PCR products, however, confirmed 29 out of 36 such SNPs genes with known single mutant phenotypes (Supplementary Table 2).

The results show that MUCH-AMASE can readily detect multiple infections, whereas conventional dideoxy sequencing resulted in ambiguous sequence.

To evaluate false positive and negative rates, dideoxy sequencing was done for 335,420 bases interrogated by the arrays.

Sanger dideoxy sequencing was performed by using BigDye Terminator v.3.1 Cycle Sequencing kits (Applied Biosystems, USA).