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We overlaid this SPOTS scan array with the purified MBP-SH3 domain of endophilin A2 that is the predominant isoform in non-neuronal cells (Ringstad et al, 1997).
We observed that the Δ ctb2 strains also caused rarely necrotic spots, but the frequency of this spots was influenced by the quality of the inoculum.
For this spots were weighted based on feature flagging in Genepix: weak signal and feature irregularity appeared to be the main source of discrepancy therefore features were flagged to avoid these spots.
On this SPOTs blot, each single amino acid of Peptide 12: TLNDKASKKIDRQ was individually substituted with L-alanine beginning with N-terminal amino acid and progressing to the C-terminal of the peptide; synthesized 13-mer peptides were fixed on a cellulose membrane.
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But why this spot?
"They surprised us yesterday at this spot.
Born on this spot 1801.
This spot tries to do just that.
I cherish being in this spot".
She deserves to be in this spot.
This spot was reachable and attainable".
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