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This 12 h period of illumination was followed by 12 h of incubation in the dark (total 24 h).
This 12 h period of illumination was followed by 12 h of incubation solely in the dark (total 24 h) also in the incubator.
At this 12 h–24 h timeframe, these cells are most likely not able to successfully exit M because of the destabilization of clients such as PLK1 [38], [38], which has roles in mitotic exit and cytokinesis [27], [50] (see Figure 7D for a timecourse analysis of PLK1 levels in XL888-treated A375 cells).
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The GPS satellites coverage are also more spread out than BDS during this 2 h period.
This 24 h infestation was repeated at least 4 times, and these guinea pigs will henceforth be referred to as 24 h tick-immune animals.
The infection efficiency did not increase further if cells were stimulated beyond this 24 h window (data not shown).
Where used, Pertussis toxin (PTX, 100 ng·mL–1) was added for this 24 h period.
This 24 H –period was compatible with pig colonic transit time.
For this, 24 h post-induction culture of C. bulleri was used for preparation of RNA.
As a precaution, data collected over this 24 h period were excluded from subsequent analyses.
Radiosensitivity was examined at this 48 h time point after transfection.
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