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Exact(57)
The swapped positions are not static.
The swapped domains of either EDS5::EDS5H or EDS5H EDS5 were constructed in pBluescript (Stratagene).
The "swapped" strategy involves replacing the endogenous FG domain with that of another Nup.
The swapped H1.1 and the native version of H1.1, both in pET11a, were expressed in Escherichia coli BL21 cells.
The swapped dimer is indeed asymmetric, with one monomer playing the role of the enzyme and the other that of the substrate.
The swapped region corresponds to the region shown to be flexible in TGF-β3 and includes α-helix 3 (residues 57 68) as well as several flanking residues.
Alternatively, servers may not predict either of the two positions for the swapped region, and evaluation over the domain core with the swapped region removed can be useful.
We found a dramatic discrepancy between corrected and precalculated predictions that is most probably explained by the swapped substitution.
Similar(3)
Starting from the random mating design, parents were randomly chosen and swapped until the MCAC mating criterion improved and then the swapped parents were retained.
A mutant at the dimer interface (C290A) disrupts the swapped-dimer formation and autophosphorylation without affecting the activity of the phosphorylated Aurora A C290A monomer.
Randomly-chosen parents were swapped until a decrease in coancestry was observed, at which point the swapped parents were retained, as in [ 5].
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