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The slides of metaphase cells or tumor sections were fixed in 4% formaldehyde before treatment with acidified 1% pepsin solution, and hybridized with probe solution (0.3 µg/mL Cy3-conjugated [CCCTAA]3 PNA probe (Panagene, Daejeon, South Korea), 70% formamide, 20 mmol/L Tris-HCl, pH 7.0, 1% BSA).
The slides of hemocytes and tissues were covered with 20 µl antibody of rCfLec-1 (diluted 1∶1000 in PBS) as primary antibody and incubated at 37°C for 1 h in a moisture chamber.
The slides of the 4 false-positives were also reviewed.
The slides of 25 patients whose diagnoses were made elsewhere were not available for review.
The slides of IHC were dewaxed and rehydrated in a gradient of ethanol and xylene.
The slides of original ovarian tumors in patients with recurrence were reviewed by pathologists in our hospital.
The slides of two cases revealed tumour deposits at review, which were missed at the first evaluation.
The slides of serial sections and the nodule containing the seed are stored in the National Museum of Nature and Science, Tokyo, Japan, as NSM-PP-9176 NSM-PP-9176 NSM-PP-9176
The slides of the remaining cases were not available, not retrieved, or of poor quality and these cases were therefore excluded from re-examination by the panel.
The slides of MCF7 cells were prepared by a cytospin method and stained with Wright Giemsa solution (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) and photographed.
The slides of hepatic tissue were soaked in 3%H2O2-methanolH2O2-methanolr 20 min in order to block endogenous peroxydase.
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CEO of Professional Science Editing for Scientists @ prosciediting.com