The IGH locus was rearranged in all cell lines (14/15 on the two chromosomes), with a specific pattern for each cell line and it expressed surface IgM.
The IgH libraries were pooled and sequenced with Illumina MiSeq pair-end read-length platform.
The IgH translocation partner remains unidentified in roughly 50% of MM cases having a 14q32 translocation.
The IGH locus displays a high level of structural complexity, with highly homologous genes arising from multiple segmental duplication events [ 26, 27].
Two patients had both the 6q deletion and IGH rearrangement, and two had only the IGH rearrangement.
(2013) provide a comprehensive dynamic view of the Igh locus architecture.
Despite the minimal mutation requirement, hybridomas carrying somatic translocations to the Igh locus were obtained.
Memory B cells of mice with Ig μ transgenes often carry transgene copies that have moved into the Igh locus via somatic translocation.
During class switch recombination (CSR), B cells replace the Igh Cμ or δ exons with another downstream constant region exon (CH), altering the antibody isotype.
Sanada returned to Tokyo on 25 December and recommended to the IGH that Guadalcanal be abandoned immediately and all priority given to the campaign in New Guinea.
Furthermore, it was suggested that the 5'end of the IgH locus might play a role in positioning the IgH locus in distinct subnuclear compartments [16], [17], [18], and it was suggested to harbor insulator or boundary capacity [19].
