We start by comparing the identified AS signals.
As cg2810 is thought to be repressed by CysR, a search for motifs similar to this inverted repeat inside and downstream of the cg2810 promoter regions was performed, identifying two candidate motifs that overlap with the identified as well as the hypothetical promoter (Fig. 4A).
Additionally, the identified AS events can be visualized and compared with predicted gene models using GBrowse for comparative assessment.
We observed that a majority of the identified AS events were related to retained introns (55.5%), followed by alternative acceptor sites (16.7%).
On average, 63% of the identified AS events predictably induce more than one functional change, with 28 predicted to induce NMD.
Among the identified AS events, intron retention remains to be the dominant type accounting for 23.5 % in S. bicolor, and up to 55.8 % in O. sativa ssp indica.
In terms of exon use, 8 of the identified AS events are described in the human genome annotation (5 C-terminus, 2 cassette-exon, 1 alt-3′ split site), and 16 involve constitutive exons.
Based on the identified AS events in Populus and Eucalyptus, AS features in woody tissues were analyzed, including AT content of the introns, and nucleotide change caused by AS.
Our systematically analyzed data provide a visualization portal to B. distachyon community researchers who aim to identify potential mechanisms of transcriptomic diversity and adaption to stress and to expand the functional relevance of the identified AS events through experimental approaches.
Ions a (cinnamyl-galloyl-glucoside derivative) and g (tR, 8.62 min, m/z 461.1085, cinnamyl-galloyl-glucoside derivative) were the identified as the most characteristic chemical markers for distinguishing between raw R. palmatum and wine R. palmatum, as well as distinguishing between raw R. palmatum and vinegar R. palmatum.
