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The HYD activity of Ni was suppressed by forming Ni-Sn intermetallic compounds by Sn addition.
The HYD reaction pathway, which involved hydrogenation to 2-hydrothiophene followed by hydrogenation to 2,3-dihydrothiophene and 2,5-dihydrothiophene, could proceed with a mild reaction barrier at the S-edge with the creation of sulfur vacancy.
The hyd mutants show enhanced ethylene responses, as well as defects in auxin responses [26], [33].
The hyd mutants exhibit ectopic lignin and callose accumulation (Fig. S1), showing a dissociation between cell patterning and wall biochemistry.
The hyd 1 ein2 and fkhyd2 ein2 seedlings (Figs. 7E, F) have greater vascular strand coherence than do fk/hyd single mutants (cf. Figs. 4D G).
5 36 Because of the higher concentration, total Na+ losses (HOT range: 1.3 4.4g/h; HYD range: 1.1 4.2 g/h) were greater in the HOT than in the HYD match.
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In addition, electron paramagnetic resonance spectroscopy of the Hyd-5 isoenzyme reveals a complex paramagnetic FeS signal at high potentials which is comparable to that observed for other O2-tolerant respiratory [NiFe] hydrogenases.
Co-ordinates for the Hyd-5 crystal structure have been deposited in the PDB under accession code 4C3O.
The Hyd-5 H229andnd LB03 E73A variants were purified by IMAC and analysed by SDS/PAGE.
The Hyd-5 structure also has strong feature in the electron density that would correspond to a hydrated Mg2+ ion.
Electrochemical analysis of the Hyd-5 H229andnd E73A variants revealed that these substitutions have dramatic effects on the catalytic properties and O2-tolerance of the enzyme.
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