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The further steps were carried out at room temperature.
The further steps involve some generally implicit assumptions: the complex concentration variation will be the sum of two parts.
The further steps of digestion and the collagen type II estimation were performed as described in the Native Type II Collagen Detection Kit 6009 protocol (Chondrex, Redmond, WA, USA).
The further steps for the 2D + 3D interpretations (Arms C and D) then included the 2D image for each view displayed on the left monitor and the 3D image for the same view was displayed on the right monitor, the cranio-caudal views first followed by the medio-lateral oblique views.
The further steps of immunocytochemical analysis have been performed as published before Dudas et al, 2011) using anti-Rac1 mouse monoclonal antibody (ab33186, Abcam) at a final dilution of 1 : 50 and Alexa 647-conjugated secondary anti-mouse antibody (Invitrogen, Carlsbad, CA, USA) at a final dilution of 1 : 1200.
The further steps towards the character state observed in tetrapods are considered to be: separation along the midline into a single midline nasal placode and a single median hypophyseal placode; and duplication perpendicular to the midline of the nasal placode into two paired, lateralized structures [ 2, 48].
Similar(20)
And Mr. Katsaris played some of those arrangements, taking the further step from, say, organ to piano.
Justice O'Connor seemed skeptical today about the further step of declaring that an unconstitutional taking had occurred.
Instead, we took the further step of providing each schoolchild with a rugged, power-efficient laptop with Wi-Fi connectivity.
In early October, McDonald's took the further step of preparing regular packets of the sauce for distribution in restaurants, and things fell apart.
Maine took the further step last year of passing its own legislation requiring the aggressive treatment of pain, and California and other states are considering following suit.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com