Exact(6)
It remains to apply Theorem 3.1 to conclude that (VRP) has a solution.
For every x ∈ [ 0, 2 ], y ∈ T ( x ) if and only if 0 ≤ y ≤ 4. With y = 4, it is clear that R ( x, y ) does not hold, which means that (VRP) has no solutions.
Although VRPs were the least effective priming modality we tested, a comparison with the Pox alone group shows that VRP priming did enhance protection and immunogenicity.
Similarly, we have shown that VRP containing either syngeneic (mouse) or human tyrosinase are immunogenic as opposed to plasmid DNA vaccination, which requires the use of xenogeneic (human) tyrosinase to overcome immunologic ignorance and/or tolerance [33].
Several reports have demonstrated that VRP are extremely effective vaccine vectors [ 28- 39].
We report here that VRP vaccine vectors derived from an attenuated strain of VEE containing the gene for rat HER2/ neu were highly immunogenic when used to vaccinate both conventional mice and mice transgenic for the rat neu gene.
Similar(54)
We found that VRP-encoded TRP-2 (VRP-TRP2) is the most effective target antigen among those tested.
To our surprise, we found that VRP-TRP2 induced very little hypopigmentation in mice, confined to the site of tumor injection.
This result clearly demonstrated that VRP- neu was much more effective for this application than ELVIS- neu and, by inference, that VRP- neu is also better than the slightly modified SINCP- neu.
These findings suggest that VRP- neu constitutes a powerful gene vaccine that induces both cellular and humoral immunity against HER2/ neu.
We have first found that a VRP vaccine targeting TRP-2 is surprisingly effective in controlling tumor growth as opposed to vaccines based on the same viral delivery vector targeting the other melanosomal antigens, gp100 and tyrosinase.
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