Sentence examples for That spb from inspiring English sources

Our observation that proteins involved in membrane insertion, such as Mps2, Bbp1, and Ndc1, also accumulate at the new SPB early in duplication suggests that SPB assembly and NE insertion are coupled events during SPB formation in wild-type cells.

To visualize the SPB pore (the protein structure that surrounds the core SPB and anchors the SPB in the NE), we exploited the fact that SPB diameter, and thus pore diameter, increases with ploidy (Byers and Goetsch, 1974; Bullitt et al., 1997).

It is postulated that SPB movement from mother cell to daughter during anaphase sends a signal to Cdc5 at mitotic exit[22].

There is certainly a precedent that SPB components may build up at the pole during a cdc20 arrest (O'Toole et al., MBoC, 1997), however, we are using this experiment to study protein accumulation at the new SPB.

These results show that SPB substructures (the inner and outer plaques) and duplication intermediates (satellite) can be detected by SIM of endogenously expressed fluorescent fusion proteins, which was not possible with previous techniques.

The increased resolution and quantitative intensity information obtained using this method allowed us to demonstrate that SPB duplication begins by formation of an asymmetric Sfi1 filament at mitotic exit followed by Mps1-dependent assembly of a Spc29- and Spc42-dependent complex at its tip.

This indicates that SPBs are absent in anucleate compartments in cdc16-116 mutant cells.

It seems equally possible that SPB-kinetochore connections must be dissociated for normal execution of prophase I events, e.g. telomere-nuclear envelope attachment, chromosome movement, etc. 5.

Our study, therefore, illuminates that SPB-mediated mitotic processes, including spindle formation, NE reorganisation, and SPB insertion converge via Pcp1 (see Figure 7C for a model); on one hand, Pcp1 recruits γ-TuC, thereby promoting nucleation and assembly of spindle microtubules; on the other Pcp1 interacts with Plo1 and is responsible for NE invagination and subsequent SPB insertion.

However, we noticed that the SPB was placed obliquely, rather than in parallel, above the NE, indicating that despite the apparent NE invagination, SPB insertion into the NE is somewhat compromised in this mutant.

From results of these tests, we found that an SPB design modified from pumpkin, named "tawara", can be a good candidate to greatly improve the deployment stability of the lobed SPB.