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Here, we present a critical evaluation of DCM in which we show that DCM can be challenged on several grounds.
Our present study expanded upon Xiao et al.'s [ 18] finding that CC-AAbs may be a risk factor for VT and SCD in a small DCM patient group and established that DCM and ICM patients positive for CC-AAbs sustained a 2- to 3-fold risk for SCD.
We show that DCM can disambiguate between alternative (neural mass and field) models of cortical activity.
It has been shown that DCM can cope with slice timing differences of up to 1 s.
Therefore we hypothesize that DCM is a disease of basement membrane, which functions to support sarcomeric interactions with the ECM, and not only impaired cardiac contractility.
The fund came together on the heels of two other funds that DCM has raised in the last 18 months, including a $170 million "Turbo Fund" that DCM is using to invest in growth-stage companies (that it mostly but not exclusively has previously funded), and a $100 million "A Fund," which is a healthy-size seed-stage fund that DCM is using to fund mobile and emerging platforms.
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The morphological analysis has revealed that DCM-DS-treated MCF-7 cells experienced cellular shrinkage, suggesting induction of apoptosis in the cells.
Western blot analysis has confirmed that DCM-DS significantly up-regulated the expression of pro-apoptotic JNK1, pJNK and down-regulated anti-apoptotic AKT1, ERK1 in MCF-7 cells.
Cell cycle analysis revealed that DCM-DS induced G0/G1 and G2/M phase cell cycle arrest in MCF-7 cells at low concentration (12.5 and 25 μg/mL) and high concentration (50 μg/mL), respectively.
The IC50 values of DCM-DS towards MCF-10A cells at 24, 48 and 72 hours were 37.6 ± 4.3, 24.0 ± 2.2 and 10.3 ± 2.1 μg/mL, respectively.Morphological study revealed that DCM-DS induced growth inhibition and apoptosis in MCF-7 cells.
Although Annexin-V/PI-flow cytometry analysis has confirmed that DCM-DS induced apoptosis in MCF-7 cells, the distinct characteristics of apoptosis such as membrane blebbing, chromatin condensation, nuclear fragmentation and formation of apoptotic bodies were not observed under microscope.
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