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During the stable operation (from days 80 to 100), mean CRE values were estimated (Table 1), showing that CRE values for ASR and MFC were not significantly different from each other.
Some studies [30, 124, 125] corroborate the results of this review and suggest that CRE may have anti-inflammatory effects.
The chapter also provides the first evidence that Cre recombinase can mediate effective site-specific deletion of transgenes in zebrafish.
These results demonstrate that Cre recombinase can be utilised in P. falciparum for deletion of specific sequences such as drug resistance genes.
The floxed region was purposefully extended so that Cre would not always recognize both floxed sites.
It is only after the addition of doxycycline that Cre is expressed.
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Examination of mouse embryos showed that Cre-mediated recombination in the developing brain caused an increase in cell death.
An explanation for this discrepancy is that Cre-mediated recombination of Hdac3 in progenitor cells is a permanent event.
This hypothesis is coherent with the observation that Cre-LoxP recombination as well as other non homologous recombination types [12], [19] are strongly inhibited in methylated regions.
It is conceivable that Cre-mediated DNA recombination can only occur when the target loxP sites are freely accessible, most likely during the DNA replication in S phase.
Notably, Cre expression in Pdx1-Cre mice is mosaic such that Cre-mediated recombination occurs far less frequently as would be suggested by the observed PDX1 expression.
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