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With the purpose of investigating their functionality, two single mutations (W164S and H232F) and one double mutation (W164S/P76H) were introduced in VPL that: (i) removed the two pathways in this isoenzyme; and (ii) incorporated the absent putative pathway.
For kinetic measurements a kinetic titration approach (Karlsson et al., 2006) was used, since no regeneration condition could be found, that (i) removed all bound DARPins from the surface and (ii) left the immobilized GFP intact.
And I, who was so happy that I left, that I took my family far away from that terrible place called Israel, that I removed them from the smell of gunpowder and blood, I sometimes feel that I'm the most miserable of all of us.
It was an out of character, knee-jerk reaction, and I felt so awful about it that I removed it".
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Our main motivation for the present study was to develop a simple and general summary of blockwise data that (i) removes the need for phase information and (ii) captures short-range linkage information, allowing more powerful inferences than the SFS.
When offered a clam off the shell, one asked that I remove some brown stuff at the base — and then ate it.
A Fitting Tribute At a recent Giants football game, a neighbor suggested that I remove my hat for the "Star Spangled Banner".
"One of the ways I figure out the significance of a character," Ms. Hershey said, "is that I remove the character from the piece.
Verizon connected me to an Apple representative, who verified that I had properly updated software before suggesting that I remove the phone's protective case, in case it was interfering with the auto-focus feature.
My hospital administrator then suggests that I remove the restraints and the catheter and reduce the doses of her sedatives so that the conditions don't get worse and our hospital doesn't lose money.
He demanded that I remove the post entirely.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com