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Test compound was replaced by DMSO (7.5% final) as control.
Test compound selection.
Test compound solutions (1.5 μl) were added to column 2 (B2-G2).
Each test compound was conducted in triplicate.
Toluene was used as the test compound.
The thrombolytic ability of the test compound is evaluated.
Tubes without any test compound were used as a control.
The test compound is injected prior to the thrombus formation.
This would indicate the anticoagulant nature of the test compound.
where Ablank is the absorbance of the control reaction (containing all reagents except the test compound) and Asample is the absorbance of the test compound.
After 12 h of incubation at 37 °C, the test compound (40 μM) was added.
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