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However, two insertions appear to have occurred at CA sites (strain NCCP11945 positions 1,577,266 and 2,185,647) and indeed some CREE have terminal CA sequences rather than TA (see Additional files 1 and 2).
To distinguish noncanonical integration from residual integrase activity mediated integration, we screened our IDLV and ICLV integration data for the presence of the characteristic 5-bp direct repeat of host DNA flanking the proviral terminal CA dinucleotide as a hallmark of integrase activity.
During the multistep reaction, the MuA present in the transpososome complex catalyzes the specific DNA single-stranded cleavages at the terminal CA dinucleotides of the L1 and R1 sites, using water as the nucleophile in such a way that 3′-OH groups of the transposable element are exposed (Mizuuchi 1984).
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With the growing trend in airport design to create satellite terminals, can't the Port Authority find a way to solve the problems of congestion and keep the T.W.A. flight center functioning as a terminal instead of an overscaled piece of sculpture?
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Protein farnesyltransferase catalyzes the modification of protein substrates containing specific carboxyl-terminal Ca(1)a(2)X motifs with a 15-carbon farnesyl group.
This domain formerly known as WA comprises different numbers of actin monomer-binding domains, also called V (for verprolin-homology) or W (WASP homology 2) and a C-terminal CA domain (connector and acidic) which activates Arp2/3 complex [11].
The C-terminal ca. 390 amino acids however shares significant identity to a class I and II aminotransferase (GenBank accession: YP_482822) from actinobacteria such as Frankia sp. This structure suggests a chimeric origin of sxtA.
These proteins carry an N-terminal sensor region, an internal DHp domain, and a C-terminal CA domain.
These results show that the C-terminal ca. 200 amino acids of hPrp3 encompass the protein elements that mediate stable U4/U6 binding.
In the N-terminal CA domain of L-CAs, the first zinc-binding histidine is replaced by arginine and the third by glutamine.
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