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Technical disadvantages are more or less the same in both methodologies; however, CC-TDI has less spatial resolution and the advantage of offline measurements.
However, it suffers several technical disadvantages due to the single recognition epitope.
This poses some technical disadvantages as the dense subchondral bone requires longer decalcification times prior to histological staining.
Trocars used during video-assisted thoracic surgery (VATS) present some technical disadvantages due to their rigid structure.
The major technical disadvantages are the limits of resolution, inability to visualize vascular structures and visualization of three-dimensional structures on a two-dimensional plane.
Currently applied perlite expansion technology suffers numerous technical disadvantages, which adversely affect product quality and limit the range of its applications.
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To overcome such a technical disadvantage, both desulfurization adsorbent and steam reforming catalyst were developed, and furthermore a fuel processing system for kerosene is evaluated by simulation.
However, Taylor criticized the game for retaining scenes from the PlayStation version that were used to conceal loading times – a technical disadvantage of optical discs that cartridges do not share.
A relatively long life span in zebrafish (3 5 years) could be viewed as a technical disadvantage for aging research, compared to such short-lived genetic animal models as C. elegans, Drosophila or even mice.
Another less technical disadvantage of this technique is the safety in the handling of radioactive products.
This indicated the possible technical disadvantage of directional RNA-seq compared with dRNA-seq to cover the 5′-ends of primary transcripts.
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