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For example, T cells might be tagged with red fluorescence, blood vessels with green, and so on.
Chillon Way: A caller reported a gate was tagged with red spray-paint in the 2900 block at 3 56 p.m. Monday.
(Supplementary Figure 2) Fluorescence microscopic analysis showed that lentiviral particles tagged with red fluorescent protein (RFP) infected IEC-6 effectively, with adequate RFP expressing in cells.
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The CD56+ NK cells were then labeled with red fluorophore-tagged CD56 monoclonal antibodies (mAbs), vigorously resuspended in 5 mM EDTA/phosphate-buffered saline (PBS) to disrupt the CD56+NK/B721 conjugates, and analyzed by fluorescence activated cell sorter (FACS).
To test this possibility, we cotransfected the EGFP-tagged TDP-43 fragments with a full-length TDP-43 that was tagged with a red fluorescent protein tdTomato (RFP).
The T cells were co-cultured for up to 4 h with the B721 transfectants, and the co-cultures were then stained with red fluorescence-tagged anti-CD3 mAbs and analyzed by FACS.
As SENP3 is a nucleolar protein, to assess the co-localization of SENP3 with CHIP in vivo, we observed cells transfected with red fluorescent protein-tagged SENP3 with and without MG132 treatment, and visualized CHIP by fluorescent isothiocyanate-labelled antibody.
To rule out off-target effects of Cdc5L siRNA, HeLa/GFP-H2B cells were co-transfected with red fluorescent protein-tagged siRNA-resistant wild-type Cdc5L or control vector together with Cdc5L siRNA.
(A) Colocalization of STX-T (RFP, RFP tagged) with TFR (green, GFP tagged) in double transfected MEFs cell.
Lissamine rhodamine-tagged lipids were integrated into the liposomes, hence marking their membrane with red fluorescence.
(D) Confocal images of live tsA-201 cells expressing hCav3.2-GFP channels (green, left column) along with mCherry-tagged CNX (red, middle column).
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