These adjustments would result in a supplementary downward shift and rightward shift in SF through Viner's (1950) framework (i.e., Figure 1 and Figure 3) and Pomfret's (1986) framework (i.e., Figure 2 and Figure 4), respectively.
For example, in the special case where k′ = 0, vertical extension becomes identical to vertical scaling, and a supplementary vertical shift could be used to adjust the baseline level to a nonzero value.
Consistent with these studies, the purified Chrimson protein showed a large pH-dependent spectrum shift (Supplementary Figure 6a and b).
In contrast, the mutation of Glu143, located outside the extracellular cavity, had minimal effects on the absorption spectrum and the pH-dependent shift (Supplementary Figure 7g).
One is a premature stop codon mutation that leads to a C-terminal truncated protein (1 254 amino acids), the other is a deletion mutation in the C-terminus further accompanied by a frame shift (Supplementary Fig. 1).
These include antisense transcription reads (to ORF-Ts), which increase in GAL compared to GLU and 25.7% of the 1772 annotated non-coding transcripts, the stable unannotated transcripts (SUTs), or cryptic unstable transcripts (CUTs), which show >threefold change in transcription on the GLU to GAL shift (Supplementary file 1E).
P CCS|AA) can be accurately estimated for any amino acid type using the probability density maps of 13C 1H correlated chemical shifts (Supplementary Figure 2).
Moreover, when URB694 (1 mg/kg) was administered along with THC, the THC discrimination function was not significantly shifted (Supplementary Figure S4A; effect of URB694 × THC dose interaction, F4, 20=1.31, p=0.30).
A heatmap for visual inspection and correction of block shifts (Supplementary Fig. S3).
The 3-NP-injected mice exhibited a normal magnitude of light-induced phase shifts (Supplementary Figure S1).
