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Such raft preparations were dissolved in 6% SDS-RIPA buffer (150 mM NaCl, 25 mM Tris HCl pH 7.4, 5 mM EDTA, 0.5% Na-DOC and 0.5% NP40).
The disruption of plasma membrane condensation at TCR activation sites by incorporation of PUFA and 7KC correlates this membrane structure with the Lo-lipid phases in model membranes suggesting that such raft lipid-phases comprise a defining element of TCR signalling plasma membrane domains.
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To appreciate the piece fully, you needed to know that in the Mongol invasion of Eurasia in the early 13th century, Genghis Khan's army forded rivers with such rafts.
But researchers had few clues to how such rafts evolved.
Our finding that such raft-like membranes, containing GPI-anchored proteins, are present within intralumenal membranes of these multivesicular endosomes fits nicely with electron microscopy observations using a cholesterol-binding toxin showing that cholesterol is abundant within these lumenal membranes [63].
Our results suggest that such rafts might be targets for therapeutic intervention.
Such rafts can be quite large, exceeding 100,000 individuals in size.
59 Such rafts play a critical role in the growth and metastasis of breast tumors.
More specifically, cholesterol is important for the formation and function of membrane rafts, and in cholesterol-depleted neurons, most of the Aβ42 was excluded from such rafts.
There has never been any river in the world with such rafting rapids.
Cell-specific parameters such as raft diameter, raft area, and receptor number may also influence receptor organization.
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