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Stepwise sampling of downstream tributaries during the late dry season confirmed the presence of Microcystis and microcystins throughout the lower Pajaro watershed to within 1 km of the ocean.
Stepwise sampling of water and surface bloom from Pinto Lake, its drainage into Corralitos Creek, and the Pajaro River that carries water from this region to Monterey Bay was performed during the bloom event.
During this same period, stepwise sampling of water and surface bloom from Pinto Lake, its drainage through Corralitos Creek and the Pajaro River confirmed the presence of Microcystis and microcystins from Pinto Lake to the river channel within 1 km of the ocean (Fig. 1).
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This procedure was then repeated at 500 angular positions of stepwise sample rotation over 180 degrees.
A standard curve analysis with stepwise sample dilution demonstrated that all primer pairs had similar efficiency (data not shown).
By application of this notion to the whole stepwise process from sampling to preparing the RNA sample and subsequent methods for normalisation, RT-qPCR will become more reproducible, which in turn will improve the impact and likelihood that findings will be translated to routine clinical use.
Validation through a stepwise increasing sample cohort and moving from severe TBI CSF to matching plasma samples and then to mTBI plasma samples will provide verification where experimental analyses and patient samples are matched with appropriate positive controls along the way.
Using all 50 clinical and laboratory variables available on day 1 of critical care admission, Cox proportional hazards regression was fitted to select variables for inclusion in two prognostic models, using stepwise selection and nonparametric bootstrapping sampling techniques.
We did our backward stepwise model by adjusting for sampling weights and clusters.
A stepwise stalagmite sample screening process was applied in order to select a stalagmite as a sensitive recorder of recent tropical cyclone rainfall events near Belize.
In detail, we started by sampling 100 of the 20,000 permutation tests and stepwise increased the number.
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