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All steps were performed at 4 °C.
This and the following steps were performed in the dark.
All steps were performed at RT, unless indicated otherwise.
The following steps were performed at 4 °C.
All subsequent steps were performed at 4 °C.
The washing steps were performed following the manufacturer's instructions.
Following passivation, all steps were performed in ATP assay buffer.
All steps were performed independently by two investigators.
All steps were performed automatically, including image selection and classification.
Afterwards, five cross validation steps were performed.
All experimental steps were performed in duplicate.
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