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The concentration of I2 standard solution, mol/L.
Calibration of the spectrometer was done, using the ICP multi – element standard solution VI by Merc.
LDH cytotoxicity was calculated according to the following formula: LDH cytotoxicity=(sample OD−blank OD)/ standard solution OD−blank standard solution OD) × 2000.
Standard solution (10 μl) was used for HPLC injections.
A 1000 mg L 1 As standard solution (Merck, Germany) was used as the iAs V) stock standard solution.
As reference, the multi-element standard solution XVI (Merck) was used.
MDA was calculated using OD as MDA level (nmol/mg protein) = (OD sample − OD blank)/(OD standard solution – OD blank standard solution) × standard solution concentration/sample protein concentration.
LDH cytotoxicity was calculated using OD as LDH cytotoxicity (U/g protein) = (OD sample - OD blank)/(OD standard solution – OD blank standard solution) × standard solution concentration/sample protein concentration.
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